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Electron Microscopic Counting of Virus Particles by Sedimentation on Aluminized Grids
[摘要] The technique described employs collodion covered grids which have been coated with a thin layer of aluminum. Negatively charged virus particles are sedimented at high speed directly upon the positively charged aluminized grid surface. Grids may be washed under a stream of water to remove soluble salts and further purify poliovirus without detectable loss of virus particles. Particle distribution is satisfactory for counting with the electron microscope. Precision of counting is quite high when substantial numbers of particles are counted, and the number of particles counted per unit area is directly proportional to the relative concentration. The relative simplicity of the method permits numerous determinations to be made within a short period of time. The method has been used satisfactorily with poliovirus, bacteriophage and vaccinia virus, three viruses differing greatly in size and morphology.
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[效力级别]  [学科分类] 生物科学(综合)
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