Analysis of Immune Precipitates by Serum-Agar Diffusion

[摘要] Anti-BSA sera obtained from chicken, guinea fowl, turkey, pheasant, duck, rabbit, rat, mouse, and guinea pig were tested for their capacity to induce coprecipitation of other serum proteins when reacted in vitro with the BSA antigen. The immune BSA-anti-BSA precipitates were analyzed by solubilizing the precipitates with excess antigen and reacting the soluble complexes against the species-specific anti-whole serum antiserum in Ouchterlony serum-agar diffusion plates. The tests have shown that, in addition to the antibody γ-globulin protein, the soluble antigen-antibody complexes of all species of anti-serum, excepting guinea fowl and rabbit, contained a coprecipitating serum protein. These results and their possible biologic significance are discussed.

[学科分类] 生物科学（综合）