An Electrophoretic Study of Antipneumococcal Horse Sera

[摘要] Electrophoretic analyses in a Tiselius apparatus have been made of several antipneumococcal horse sera and of concentrates produced from them. In every instance the antibody lay in the γ-component. Thus though pneumococcal horse antibodies are for the most part larger than the molecules of normal γ-globulin they display essentially the same electrical behavior.Unsuccessful attempts were made to resolve antibody and γ-components by the prolonged electrophoresis of a serum both diluted and undiluted and by electrophoresis at different pH values.Measurements of areas on Longsworth's electrophoretic diagrams of sera before and after absorption with specific polysaccharides demonstrate that antibody-content estimated in this way agrees well with that determined chemically.

[学科分类] 生物科学（综合）