Isofixation Curves As a Method of Standardizing Quantitative Complement-Fixation Tests

[摘要] The purpose of the present report is to call attention to the “isofixation curves,” which can be constructed from a box titration of serum and antigen in the presence of fixed amounts of complement, and the value such curves may have in defining the relationships between antigen and antibody in a variety of complement-fixation systems. The principles have been implicit in earlier work[1][1] but their full usefulness may be greater than has been appreciated.Materials and Methods. Sera . The sera used in each test are indicated in the figures. Sera were inactivated at 56°C for 30 min and dilutions were made in normal pooled sera or saline.Antigens . The antigens were prepared according to specifications given elsewhere (2–12). Dilutions were made in borate salt solution, pH 7.6 (13).Complement . Pooled guinea pig sera titrated in 50% hemolytic units with maximally sensitized sheep cells were used. [1]: #fn-1

[学科分类] 生物科学（综合）