Lymphoid Precursors: Thymus Independent Antibody Production

[摘要] Thymectomized and nonoperated adult mice were lethally irradiated and given liver cells from embryos of various ages. The resultant chimeras were hyperimmunized with sheep erythrocytes, and cell suspensions from their spleens were used in a hemolytic plaque assay employing specific anti-allotype antisera as developing agents in order to distinguish between cells producing host and donor-type γG2a (7S) antibodies. It was found that lymphiod stem cells taken from embryos as early as the 10th day of gestation (i.e., before the appearance of the thymic rudiment) were able to produce γG2a anti-SRBC antibodies in thymectomized hosts. The number of donortype “developed” plaques found in the spleens of thymectomized hosts was approximately one-tenth that found in their nonoperated controls.Sensitization of the hosts to SRBC before irradiation and the transfer of embryonic liver cells did not increase the number of donor-type PFC.

[学科分类] 生物科学（综合）