In Vitro Immune Responses of Rabbits with Persistent Adenovirus Type 5 Infection

[摘要] Antibody responses of rabbits to adenovirus type 5 were studied. Serum antibodies were still present 12 months after the animals were given a single injection of infectious virus. Isolated lymphoid tissues from these animals were capable of synthesizing high antibody titers in vitro if exposed to viral antigen prior to cultivation. Antibody-producing cultures were maintained up to 120 days. Serum antibody titers of animals immunized with the soluble antigens declined rapidly. Synthesis of antibody could be inhibited by the addition of chloramphenicol and puromycin to the culture media. Phytohemagglutinin enhanced antibody synthesis of established antibody-producing cultures. The antibody species synthesized in vitro was primarily 7S although some 19S antibody was produced. Evidence for a persistent infection in the lymphoid tissues of rabbits given infectious virus was obtained by recovery of the virus from the tissues of the animals up to 12 months after the initial injection. The virus was also isolated from spleen and lymph node fragments maintained in vitro . This study indicates that persistent infection of the rabbit with adenovirus type 5 was responsible for the prolonged immune response.

[学科分类] 生物科学（综合）