Immunization with an Induced Avirulent Auxotrophic Mutant of Pseudomonas Pseudomallei

[摘要] A virulent strain of Pseudomonas pseudomallei was subjected to ultraviolet irradiation and a mutant clone requiring preformed adenine or hypoxanthine for growth was isolated. The imposition of auxotrophism was associated with a striking decline in virulence for mice which could be restored by permitting the strain to revert to purine independence in vitro . The mutant persisted in mice without detected reversions or or untoward effects for approximately 20 days following inoculation of 107 cells. After repeated inoculations, a significant immune response was demonstrated by parenteral challenge with diverse strains of this species. However, animals so immunized remained susceptible to lethal respiratory infection.

[学科分类] 生物科学（综合）