In VitroUptake by Rat-Tissue Homogenates of Radionated, Purified Antirat-Organ Serum

[摘要] Rat-lymph node or rat-kidney homogenates were injected into rabbits to stimulate production of antibodies against the tissue used. The giobulin first obtained from the immunized rabbits was radioiodinated and “purified ” by adsorption on and elution from rat-lymph node homogenate or rat-kidney homogenate respectively. The resulting solution was tested in t~tro by adsorption tests against various organ homogenates. There was more radioactivity in the precipitates formed with rat-lymph node homogenate or with rat-kidney homogenate, respectively, than in the precipitates formed with other organ homogenates.The solution derived from rabbit antiratlymph node serum contained a labeled component which appeared to be specifically adsorbed by rat-lymph node homogenate. The solution derived from rabbit antirat-kidney serum contained a labeled component which appeared to be specifically adsorbed by rat-kidney homogenate.There were several cross-reactions.

[学科分类] 生物科学（综合）