In Vivo Purification of Isp131 Labeled Localizing Antirat Lymphosarcoma Antibody

[摘要] Antibody preparations were separated from the sera of rabbits immunized with a homogenate of rat Murphy lymphosarcoma plus Freund adjuvant. When labeled with I131 and injected intravenously into rats bearing this tumor, they showed substantial tumor localization. One day after intravenous injection the portion of the injected I131 found in the tumors of 20 rats averaged 6.8% of the injected dose in an amount of tumor equal to 1% of the animals' weight. This I131 concentration in tumor was more than twice the blood concentration at time of sacrifice and several times the concentration found in any other perfused organ or tissue. The tumor localizing substance behaved like γ-globulin with starch electrophoresis. Control studies with labeled normal γ-globulins indicated that most of the accumulation of I131 in tumor was specific and due to antibody to the Murphy tumor.The antibody purification procedure involved a step in which a labeled crude antibody preparation was injected into tumor bearing animals, the animals sacrificed a day later, and the I131 labeled antibody attached to the washed homogenate of these tumors eluted into solution with 32% urea in pH 8 borate buffer.Preparations made by completely in vitro methods gave less specific tumor localization.

[学科分类] 生物科学（综合）