In Vitro and In Vivo Studies on a Tissue Culture Cell Line Derived from Normal Monkey Heart

[摘要] The SCH tissue culture cell line derived from cynomolgus monkey heart by Salk and Ward (1) was found to grow vigorously in serial tissue culture. Data are presented on its growth characteristics, microscopic appearance, mitotic index, adhesion to glass, abnormal mitoses, chromosome number, susceptibility to infection with poliovirus Types 1, 2, and 3, and to adenovirus Types 3, 4, and 7, serologic relationship to human and monkey cell antigens, and results of transplantation into normal and into cortisone-x-ray treated monkeys (10 animals) and Wistar rats (171 animals). These studies suggest that the SCH cell has characteristics which make it a valuable addition to tissue culture technology. Large scale production of polioviruses in this cell is feasible. However, the SCH cell produces fatal tumors in specially prepared Wistar rats and careful evaluation of this property is indicated before the cell line can be used in preparation of biologic products intended for human use.

[学科分类] 生物科学（综合）