Biochemical Studies of Bacterial Derivatives

[摘要] Human tubercle bacillus protein MA-100 was isolated by Masucci and McAlpine (1) in November, 1929, from the filtrate of cultures of the human tubercle bacillus H-37, grown on Long's synthetic culture medium. This medium contains no protein, but contains asparagin as a source of organic nitrogen. All proteins present with various other substances, not medium constituents, therefore, in the filtrate must have been synthesized by the tubercle bacillus.One of the most important of these substances is a specific polysaccharide, closely associated with the proteins. However, Masucci and McAlpine have been able to isolate not only a protein MA-100 free from the polysaccharide, but to obtain the poly-saccharide free from the protein. The designation MA-100 is given the protein to classify the material with the chart published by Johnson (2).Only one phase of these investigations; namely, the skin sensitivity of man to this particular human tubercle bacillus protein MA-100, when the protein is introduced intradermally, is dealt with in this communication.

[学科分类] 生物科学（综合）