The Relationship of Two Hemotoxic Antigens in B. Welchii Growth Products

[摘要] In the sterile filtrates of B. welchii broth cultures two hemotoxins have been demonstrated. One of these designated as A is active in vitro and probably to some extent in vivo ; the other, B, causes no hemolysis in vitro but marked blood destruction in vivo . These two principles were not completely separated.The hemotoxin A (hemolysin) was produced in such strength that 0.0075 cc. completely lysed 1 cc. of 5 per cent washed red blood sheep cells in eighteen hours.Hemotoxin B was generally found to be much more toxic for pigeons than A.Immune sera were produced with these two hemotoxins. It was found that anti-hemotoxin A serum in high dilution would neutralize its homologous antigen whereas anti-hemotoxin B had no effect on it.On intravenous inoculation into rabbits it has been found that hemotoxin B alone caused approximately as marked an anemia as a mixture of A and B. Although A apparently gave rise to a more rapid initial hemolysis in vitro than B, the continuing anemia and morphological blood changes were evidently due to the action of hemotoxin B.

[学科分类] 生物科学（综合）