The Growth Curve of the Virus of Herpes Simplex on the Chorioallantoic Membrane of the Embryonated Hen's Egg

[摘要] An estimate of the accuracy of methods of titration of the herpes simplex virus on the CAM has been given. The pattern of growth of the herpes virus on the CAM has been described, and its similarity to the patterns of other animal viruses studied has been indicated.Initial adsorption of virus onto the CAM occurs very rapidly, 99% or more occurring within 1 hour, with most of it within 10–15 minutes. Following this there is a continued decrease in residual seed virus at a slower rate for the next 7 hours.The latent period varies in length (from 4–12 hours) inversely with the number of infectious units in the inoculum. During this period there is no detectable evidence of infectious virus in the host cells.Following the latent period infectious virus becomes again detectable and increases at the rate of 10 fold every 2 hours for the next 10 hours. Following this, the rate levels off for another 6 hours, then rises again slowly.For the first 12–18 hours of the incremental period more virus is obtained from within the membranes than can be demonstrated as having escaped from the infected cells. After this period, virus formation within the cells appears to come into equilibrium with the escape of virus from the cells.No evidence of complement-fixing activity was found before the demonstration of infectious virus in high titer.

[学科分类] 生物科学（综合）