The Preparation of 0.1 L+ Toxin-Antitoxin Mixture

[摘要] The media for diphtheria toxin to be utilized for toxin-antitoxin mixture is prepared in the usual way with the following exception: To every pound of ground fat free veal a liter of water is added and placed in a refrigerator over night. The following morning the liquid is collected and boiled. The media is boiled at this stage to kill all organisms except the few heat resisting spores, thereby practically limiting the bacterial protein soluble substance to the coli and diphtheria bacilli which are later added. After boiling the reaction is corrected to 1.5 acid to phenolphthalein and 10 cc. of a twenty-four hour culture of B. coli is added for every liter of media and incubated about eighteen hours. One egg for every 2 liters is added, then boiled and strained. The reaction is corrected to 0.5 acid to phenolphthalein.

[学科分类] 生物科学（综合）