Studies on Acute Respiratory Infections

[摘要] Barnes, (1), in a study on the cultural and serological relationship of hemolytic streptococci reported recently that the serums of rabbits intensively immunized against the streptococcus will give precipitin reactions with streptococcus antigens even when such serums are diluted as high as 1-3200.His method of eliciting what he terms a precipitin reaction, was as follows: The streptococci were grown in broth for forty-eight hours at 37°C. The broth cultures were centrifuged at high speed to sediment the cocci. In each test a constant amount (1 cc.) of clear undiluted supernatant broth antigen was added to an equal amount of the diluted antistreptococcus serum. These mixtures as well as controls with normal rabbit serum and with uninoculated broth were incubated at 37°C. for eight to ten hours, removed to the ice-box for two hours and then read. Sterility tests were made to eliminate the possibility of the precipitate being bacterial growth.

[学科分类] 生物科学（综合）