Observations on the Agglutinative and Hemolytic Action of Calf Serum on Sheep Cells

[摘要] 1. 1. The sera of four calves were found to agglutinate sheep cells. One of these sera produced strong hemolysis of sensitized sheep cells and partial hemolysis of unsensitized cells. The serum from one of the other calves produced slight hemolysis of both sensitized and unsensitized cells.2. 2. The addition to the active calf serum of cytozyme in the forms of blood platelets caused the formation of a second clot.3. 3. The serum from this second clot did not agglutinate or hemolyze sheep cells, either sensitized, unsensitized, or unsensitized in the presence of complement.4. 4. The addition of anti-coagulants, sodium citrate or oxalate, to the mixtures of active calf serum and cells prevented the agglutination of the latter and completely inhibited their hemolysis except in one instance in which the citrate was mixed with active serum, unsensitized sheep cells and complement. Only partial inhibition was obtained with this mixture.5. 5. Removal of the blood platelets from the sheep cell suspension reduced but did not remove the agglutinative action of the calf serum and did not affect the hemolysis.

[学科分类] 生物科学（综合）