Action of Anticellular Sera on Virus Infections

[摘要] Various heated anticellular sera were tested for cell agglutination and for a protective effect against viruses in 23 different stable cell lines and five types of trypsinized primocultures derived from several animal species.The tests indicate that HeLa cells and all other human cell lines susceptible to poliovirus, as well as Salk's monkey heart cell line, belong to one homogenous antigenic group and that cross-reactions between these lines are complete.It is assumed that these lines share two antigens in common: an antigen linked to susceptibility to poliovirus and an antigen present in human and monkey cells. As evidenced by partial or weak cross-reactions, either of these two antigens may exist separately in some stable lines or primocultures, e.g. , the antigen linked to poliovirus susceptibility in Westwood's rabbit lines; a human antigen, very similar to a monkey antigen, in human and in monkey cell lines which are not susceptible to poliovirus. Cultures not of human or monkey origin and not susceptible to poliovirus had no antigenic relationship to human cell lines or to monkey kidney primocultures (with one exception).In contrast to those human cell lines which are susceptible to poliovirus (prototype, HeLa), the human cell lines which are not virus susceptible do not form a homogenous group. They do share some human antigen in common but seem to contain another distinct antigen specific for each line.During the first week of cultivation, trypsinized monkey kidney cultures seem to acquire a new antigen which is present in cultures of the HeLa group but absent in the noncultivated monkey kidney organ.Homografts of monkey kidney will support multiplication of poliovirus. Antisera prepared against these grafts exhibited a strongly protective effect on MK cultures infected with virus. Antisera prepared against kidney suspension were only weakly active. It is possible that homografts have an ability, like that of MK cultures in continuous cultivation in vitro , to elaborate the antigen linked to virus susceptibility.Within the HeLa group, some lines displayed more susceptibility to agglutination by various antisera than did other lines. Chang's human liver line (HL) was particularly susceptible to agglutination but, conversely, was less easily protected against virus. However, anti-HL sera had weak agglutinative and high protective effect. No explanation for this last phenomenon is offered.

[学科分类] 生物科学（综合）