The Agglutination of L Cells by Vaccinia Virus

[摘要] The results of studies on the L cell-vaccinia virus interaction indicated that the L cell is relatively resistant to both infectious virus growth and release. Although low doses of virus can infect the cells, only higher multiplicities of infection induce the agglutination reaction. This reaction is independent of infectious virus growth and perhaps of noninfectious virus synthesis; it is dependent on temperature and on glycolytic cell metabolism, but independent of cell metabolism inhibited by azide and amethopterin. Agglutinated cells remain alive for 3 days and can partially reagglutinate during this time after they are first separated by a proteinase. Both the reagglutination and agglutination reactions have similar temperature and metabolic requirements.A greater (cohesive) force can be observed to act in infected cells undergoing agglutination or reagglutination than is observed in uninfected cells which are allowed to clump under certain conditions.It is suggested that the virus by the act of adsorption and/or penetration induces cell rounding but permits the cells to produce or maintain an otherwise normal substance which has caused the cells to become more cohesive (agglutinated) than adhesive (to glass) because of infection.The mechanism of L cell agglutination is different from myxovirus induced hemagglutination.

[学科分类] 生物科学（综合）