The Similar Specificity of the Combining Sites of an Individual Antibody Molecule

[摘要] Antiserum prepared in rabbits against bovine-γ-globulin (BGG) to which p -azobenzoate groups were coupled contained precipitins directed against BGG and against the haptenic group. Treatment of a γ-globulin fraction of the antiserum with an excess of a solid adsorbent capable of removing antibody with anti-BGG combining sites did not decrease the number of antihapten sites. The failure to remove antihapten activity was shown by direct binding measurements with labelled p -iodobenzoate.It is concluded that essentially none of the antihapten combining sites are on molecules having an anti-BGG site; i.e. , that heteroligating antibody is absent. The results are discussed from the standpoint of the mechanism of antibody formation.

[学科分类] 生物科学（综合）