Antigen-Antibody Reactions in Agar

[摘要] In the Oudin serum agar technique, at sufficiently low antigen (Ago) and high antibody (Ab), Oudin showed empirically that the equation k = m (log Ago - log R′ Ab) holds. In this equation, k is the slope of the straight line obtained by plotting x , the distance which the leading edge of the band moves in time, t , against t ½. An equation of this same form had been derived previously in which m was equal to 4.08 √ DA . This led to the belief that m might be proportional to √ D where D is the diffusion coefficient of the antigen. This possibility was confirmed when it was shown that the increase of nonspecific protein affected m in the same way that an increase of nonspecific protein would affect D . In addition a direct proportionality between m and √ D for egg albumin, human serum albumin, bovine γ-globulin, thyroglobulin and hemocyanin was demonstrated. Some possible uses for and limitations of the determination of m were briefly discussed.

[学科分类] 生物科学（综合）