Antibody Synthesis by Homotransplanted Cells and Tissues

[摘要] By attention to technical details, particularly the number of spleens employed and the time of removal of the donor spleens, the transplantation of splenic cells from toxoid-immunized rats into recipients consistently resulted in antitoxin production. Splenic fragments caused greater antibody formation than homogenates or cells. Antigen could be added to splenic cells from primarily stimulated donors, the cells washed and antibody be formed upon transfer. Transfers into pantothenic acid deficient recipients of cells removed from 1 donor 7 days after the booster eventuated in antibody synthesis, whereas transfers into normal rats did not. Similar cell transplants from donors 7 day-s after secondary injection resulted in equivalent antibody production in deficient and normal recipients. Usually there was a drop in titer 3 days after the booster inoculation, and an increase 7 days after this inoculation. The rat produced abundant amounts of diphtheria antitoxin, but no antibody was detected by precipitation or hemagglutination methods when bovine γ globulin was injected. The organ sites of antibody formation as related to the routes of injection of donor were similar in rat and rabbit. In no instance did a recipient which produced antibody after transfer show an anamnestic reaction to reinjection of toxoid 3 to 4 weeks after transfer. Most of the transplanted cells were lymphocytes. Interrelations, explanations and implications of these data were discussed.

[学科分类] 生物科学（综合）