Antigenic Properties of Human Mucous Membranes

[摘要] 1. 1. Human respiratory mucosa crude extract was fractionated by ammonium sulfate precipitation, starch block electrophoresis and agar gel electrophoresis. The serologic reactions of these fractions were studied by means of immunoelectrophoresis, double diffusion in agar and tanned cell hemagglutination.2. 2. The respiratory mucosa fractions precipitated by 0 to 1.34 M ammonium sulfate (Fraction I), 1.34 to 2.00 M ammonium sulfate (Fraction II), and 2.00 to 3.00 M ammonium sulfate (Fraction III) all produced precipitation with specific antiserum to bronchial mucosa.3. 3. Lung was similarly fractionated and showed visible precipitation lines in Fraction II and Fraction III using the same bronchial mucosa antiserum.4. 4. By means of double diffusion in agar all the lines formed by the various fractions of respiratory mucosa showed reactions of identity. The faint lines formed by fractions of lung were partially identical to those formed by fractions of respiratory mucosa.5. 5. Tanned cell hemagglutination tests were positive in highest titers with Fractions I and II of respiratory mucosa, Fraction II giving a lower titer. The remaining fractions did not react in hemagglutination tests.6. 6. Inhibition of hemagglutination of Fraction I-coated cells and Fraction II-coated cells was shown by Fractions I, II and III of respiratory mucosa. Fraction III inhibited agglutination of Fraction II-coated cells in greater dilution than did Fraction II itself. No significant inhibition was demonstrated by extracts of lung, esophagus, or kidney.7. 7. Different levels of the respiratory tract differ quantitatively in their content of the antigens represented in Fraction II.

[学科分类] 生物科学（综合）