Immunochemical Studies of Hemocyanin from the Giant Keyhole Limpet (Megathura Crenulata) and the Horseshoe Crab (Limulus Polyhemus)

[摘要] A method of obtaining a highly purified preparation of horseshoe crab hemocyanin which was found to be highly antigenic in rabbits is described.The horseshoe crab hemocyanin migrated as a single band by cellulose acetate electrophoresis at pH 7.0 and 7.5 and showed a single line of precipitation with rabbit anti-HCH serum in agar-gel diffusion studies.Cross-reactivity between the hemocyanins of the horseshoe crab and the giant keyhole limpet by quantitative precipitation, and by immunogenicity tests both in vitro and in vivo , were found to be negative.

[学科分类] 生物科学（综合）