Surface Properties of the Egg and Mouse Lines of A1 Influenza Virus

[摘要] Changes in the surface properties of a viral population were studied in the course of adaptation of A1/Moscow/1949 influenza virus to mouse lung. The mouse line virus (Em2), as compared with egg line virus (Em1), was found to be non-sensitive to both α and β inhibitors. Heating at 56°C for 30 min converted the Em2 virus to the “indicator” state, while heating at 62°C caused complete destruction of its hemagglutinating ability. The hemagglutinins of mouse virulent virus (Em2) were highly sensitive also to treatment with trypsin or papain. On the other hand, the immunogenicity and antibody-binding capacity of Em2 virus were higher and were independent of the presence of thermolabile components in sera which seem to be necessary for the reaction of egg line virus (Em1) with early antibodies in hemagglutination-inhibition tests. The two lines of virus differed also in their ability to agglutinate bovine erythrocytes at room temperature. Some of these markers were tested in various other strains of myxovirus. Attempts were undertaken to obtain “pure” populations of the Em1 and Em2 lines by passaging the egg line virus in the presence of trypsin and the mouse line virus in the presence of normal sera in eggs. The results of these studies are discussed.

[学科分类] 生物科学（综合）