I. Reverse Anaphylactic Reactions in Guinea Pig Ileum Strips Passively Sensitized in Vitro with Various Albumin Antigens

[摘要] Reverse passive anaphylactic reactions were induced in guinea pig ileum strips sensitized in vitro with various albumin antigens. Guinea pigs from strains highly susceptible to direct anaphylaxis regularly gave reverse anaphylactic reactions. However, with use of a method of sensitization in a low ionic strength medium (isotonic buffered glucose (IBG) solution), reverse anaphylactic reactions could also be regularly induced in ileum strips of guinea pigs from various strains less susceptible to direct anaphylaxis.Whatever may be the sensitizing medium (Tyrode's or IBG solution), reverse anaphylactic reactions are governed by the same quantitative conditions as the direct reactions, namely, a ) the antigen concentration in the sensitizing medium, b ) the length of incubation time with antigen and c ) the amount of antibody used to elicit reverse anaphylactic contraction.In contrast to γ-globulins, ovalbumin was found to be readily washed out of the sensitized ileum strips. Washing of ovalbumin-sensitized ileum strips for 10 to 20 min is sufficient for “desensitization.”As in direct passive anaphylaxis, only antibodies originating from certain species are capable of eliciting reverse anaphylactic reactions: horse antibody was not capable of producing anaphylactic contraction in conditions where rabbit antibodies were active.Nonspecific γ-globulins, when mixed with rabbit antibody for challenge, inhibited the reverse anaphylactic contraction of ileum strips sensitized with ovalbumin.The evidence concerning the correspondence of this reversed procedure with the reversed reaction at the tissular level and the theoretical implications of the reported findings are discussed.

[学科分类] 生物科学（综合）