Immunochemical Studies on Polysaccharides of Yeasts

[摘要] Quantitative precipitin reactions have shown that: 1. 1. Antiserum to C. albicans Group B reacted strongly (80 to 120% of the homologous reaction) with polysaccharides from C. albicans group A, C. stellatoidea, C. tropicalis, H. capsulata and T. pinus ; mannans from S. cerevisiae and S. pini precipitated approximately 55% of the antibody; and polysaccharides from H. minuta, H. holstii and C. laurentii var. flavescens less than 10%.2. 2. Antiserum to C. stellatoidea reacted strongly with C. albicans group B and C. tropicalis mannans (80 to 100%); polysaccharides from C. albicans group A, S. cerevisiae , and H. minuta precipitated 36 to 44% of the antibody; S. pini and H. capsulata , 18% of the antibody; and those from C. laurentii var. flavescens, H. holstii and T. pinus , less than 10%.3. 3. C. tropicalis antiserum reacted strongly with C. albicans group A and B (91 to 103%); phosphomannan from H. capsulata removed 74% of the antibody; mannan from S. pini , 45%, and all other polysaccharides, less than 10%.Double diffusion reactions in agar showed that all Candida mannans and C. albicans group B or C. stellatoidea antisera produced precipitin lines that fused. Using C. tropicalis antiserum, spur formation was observed between the homologous and group B and C. stellatoidea mannans. Of the other polysaccharides that formed precipitates in agar, all showed spur formation with the homologous mannan.

[学科分类] 生物科学（综合）