Purification of Highly Conjugated Precipitins and Globulins Containing Reagins with Silk Hydrolysate

[摘要] Highly conjugated human globulins containing reaginic antibodies against egg albumin, ragweed pollen and cottonseed and conjugated rabbit precipitins against egg albumin and ragweed pollen were purified by adsorption with silk hydrolysate. The purified antibodies were still highly conjugated and gave satisfactory specific staining of antigen in allergic human skin. The reduction in nonspecific staining was probably due to adsorption of noncovalently bound dye by the silk hydrolysate.The presence of antibody-bound antigen in epithelial cells of challenged, allergic skin was confirmed. The epithelial cells of histamine-challenged, allergic skin were capable of binding specific antigen when this was supplied in vitro .The human reaginic globulins and the rabbit precipitins gave similarly effective staining and inhibition results and the localization of antigen demonstrable with either antibody was the same.

[学科分类] 生物科学（综合）