The Antigenicity of Alkali-Treated Bovine Serum Protein

[摘要] 1. 1. Alkali-treated proteins were prepared by digestion of bovine serum with 1 N sodium hydroxide solution at room temperature for various periods of time up to 24 hours. The alkali-treated proteins were recovered from the digests by isoelectric precipitation. These proteins differed in the ease with which they could be dissolved in slightly alkaline solution. Bovine serum protein exposed to alkali for 5 minutes was rendered insoluble under these conditions.2. 2. Serological and toxicological studies with these derived proteins revealed that, with increased exposure to the alkali, the protein first lost its capacity to produce specific antibodies; then lost its antigenic capacity but retained its power to act as a hapten; and finally lost its haptenic capacity, thus becoming non-antigenic but simultaneously becoming toxic.

[学科分类] 生物科学（综合）