Studies on Chemical Nature and Standardization of Pollen-Antigen

[摘要] 1. 1. It has been re-emphasized that the pollen-antigen is destroyed by alkalies and oxidation, but is not destroyed by acids.2. 2. It has been shown that when concentrated HCl is added to ragweed-pollen-extract it combines with the antigen to form the hydrochloride which precipitates out. This reaction provides a means of separation and purification of the pollen-antigen.3. 3. Analysis of this purified antigen, shows it to have a minimal molecular weight of 4453 as determined by its sulphur-content. It contains 36 nitrogen-groups, one SH group and is able to combine with 10 molecules of HCl.4. 4. The pollen-antigen-molecule is pictured as containing 10 basic groups loosely combined with some other substance. This other substance is a hapten or a residue antigen.5. 5. The purified pollen-antigen is levo-rotatory.6. 6. An X-ray picture of the pollen-antigen hydrochloride indicates it to be a complex molecule.7. 7. It is believed that the pollen-antigen is a polypeptide which contains a large per cent of the basic amino-acids (arginine, histidine, lysine).8. 8. It is shown that 0.0022 microgram of pollen-antigen hydrochloride nitrogen equals 1 Noon unit and 0.0033 microgram equals 1 protein-nitrogen unit.

[学科分类] 生物科学（综合）