The Effect of Homologous Antiserum and Complement on the Multiplication of Vaccinia Virus in Roller-Tube Cultures of Blood-Mononuclear Cells

[摘要] 1. 1. The virus of vaccinia may be cultivated for at least 3 weeks in roller-tube cultures of mononuclear cells obtained from the circulating blood of the rabbit.2. 2. Following inoculation of the virus, the addition of homologous antiserum to this system does not bring about permanent inactivation of the virus.3. 3. Mixtures of antiserum and virus giving no reaction in the rabbit's skin give rise to an infection of these mononuclear cells. The infection is maintained even if further amounts of antisera are subsequently added to the culture.4. 4. The addition of 1 or 2 hemolytic units of complement in the form of fresh normal rabbit-serum does not enhance the effectiveness of the antiserum.5. 5. In cultures of mononuclear cells, the virus does not increase as rapidly nor reach as high a titer as in cultures consisting principally of fibroblasts.6. 6. In cultures of mononuclear blood-cells, complement and antiserum appeared to be more effective in temporarily suppressing the activity of the virus than in cultures consisting principally of fibroblasts.7. 7. The possible application of these observatins in vitro to the dissemination of virus through the animal body and to the maintenance of latent infections is discussed.

[学科分类] 生物科学（综合）