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Experiments on the Removal of Hemagglutinin from Rabbit Antihuman Serum
[摘要] In conducting the complement fixation test for syphilis with an antihuman hemolytic system, agglutination of the erythrocytes not infrequently interferes with hemolysis and the frequency and intensity of agglutination constitutes a drawback in the usefulness of the antihuman system.One of the sources of this agglutinin is the immune rabbit hemolytic serum and at the suggestion of Professor Kolmer, we have conducted a series of experiments with rabbit immune sera with the object of determining whether or not agglutinin for human cells may be removed without disturbing the hemolysin content. Professor Kolmer has been of the opinion that drying antihuman rabbit serum on paper after the method of Noguchi, resulted not only in facilitating the preservation and manipulation of the hemolysin but also in the deterioration of agglutinin without commensurate destruction of hemolysin and that this constituted an important reason for the use of paper amboceptor in complement fixation tests when an antihuman hemolytic system is employed.
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[效力级别]  [学科分类] 生物科学(综合)
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