The Relationship of Sulfhydryl and Disulfide Groups to the Antigenic Power of Bovine Serum Albumin

[摘要] 1. 1. Bovine serum albumin has been treated with several reagents which chemically alter the sulfhydryl and disulfide groups, although other effects have not been excluded.2. 2. With the alteration of these groups by each of the methods described below, bovine serum albumin loses its antigenic power as measured by its capacity to produce anaphylaxis in guinea-pigs.3. 3. The sulfhydryl and disulfide groups of bovine serum albumin have been oxidized with iodine to yield products containing no detectable disulfide groups. These products behave like the disulfoxide of cystine in that they undergo dismutation in neutral solution with formation of disulfides.4. 4. Condensation of electrolytically reduced bovine serum albumin with pyruvic acid yields a product which gives no nitro-prusside test in guanidine solutions, but does give a strong reaction after treatment with alkaline cyanide.5. 5. Oxidation of the above condensation product with hydrogen peroxide oxidizes the sulfur to a group which is stable to alkaline cyanide, possibly a sulfone.6. 6. The oxidized condensation product is more soluble and more completely despeciated than the oxidation products obtained with iodine. It does not undergo the dismutation shown by iodine or peroxide-oxidized albumin in neutral aqueous solution.

[学科分类] 生物科学（综合）