Studies on Brain Antigens

[摘要] A water-soluble bovine antigen specific for nervous tissue, α-BASNT, has been purified by a series of steps involving ammonium sulfate precipitation, DEAE-cellulose chromatography and preparative electrophoresis. The antigen also occurs in rat and human brain and in bovine spinal cord, but not in other bovine organs or in rat peripheral nerve. It is formed early in the development of the brain, as it was present in the brain of a 3-month-old human fetus. The cytoplasm of glial cells is rich in α-BASNT.α-BASNT has a molecular weight of about 84,000, formed one band when analyzed at pH 8.9 or 7.2 in gels containing different concentrations of polyacrylamide, and in agar gel immunoelectrophoresis α-BASNT had the electrophoretic mobility of an α globulin. It contained 2% carbohydrate, and, although only slightly acidic, glutamic and aspartic acids composed 23% of its total amino acid content.Differences between α-BASNT and other brain proteins that have been identified within the last decade are discussed.

[学科分类] 生物科学（综合）