Detection of Hemopoietic Allografts by Localization of Radiolabeled Alloantibodies

[摘要] Radiolabeled alloantibodies have been used to show the presence of allogeneic cells in marrow chimeras. Alloantigens of grafted cells are available to circulating antibody since radiolabeled alloantibodies bind specifically to the cells after intravenous injection. Such antibodies localize in livers and spleens of untreated allogeneic mice and of irradiated mice bearing a bone marrow graft. The presence of allogeneic cells, as determined by localization of radioantibody, was shown 18 hr after injection of the radiolabeled antibodies. Maximum localization of the C57BL/6 anti-DBA/2 alloantibodies injected could be shown 2 days after transplantation of 2 × 107 DBA/2 marrow cells into irradiated C57BL/6 or C57BL/10 mice. Similar localization occurred also 9 days after transplantation of 5 × 106 cells. The results suggested that even smaller numbers of grafted marrow cells could be detectable at shorter intervals after transplantation by this sensitive method.

[学科分类] 生物科学（综合）