Preparation and Use of Rh Testing Sera

[摘要] The existence of an antigen in the erythrocytes of rhesus monkeys, and accordingly designated as Rh, which is found also in the red blood cells of most human beings, was reported in 1940 by Landsteiner and Wiener (1). Methods for preparing anti-Rh sera in experimental animals and of testing for the antigen in erythrocytes are described by these same workers (2). It has been shown, also, that Rh agglutinins may appear in the blood serum of certain individuals who are devoid of the antigen, and that this appearance may be associated with disorders of clinical importance, e.g., transfusion reactions and erythroblastosis fetalis (3, 4). In connection with our study (5) of these problems we have employed antisera from animal and human sources in testing for the Rh antigen. In the present communication are reported our experiences in the production of animal sera and in the absorption of these sera for the purpose of enhancing their usefulness.

[学科分类] 生物科学（综合）