The Preparation of the Type-Specific Carbohydrates of Gonococci

[摘要] The observations, published in a preceding article, concerning skin reactions, with the polysaccharide fractions of type I and type II gonococci are supplemented, on the basis of new investigations, by a detailed account of the technic of the production of these substances and the difficulties encountered.With type I gonococcus the protein and carbohydrate are apparently precipitated at approximately the same level of acidity. By repeated alcohol precipitations the water-insoluble protein, accountable for non-specific skin reactions, was generally eliminated and a satisfactorily purified type-specific polysaccharide fraction obtained. Purification of type II was easier.A method based on tryptic digestion proved unsatisfactory.

[学科分类] 生物科学（综合）