The Separation of a Protective Antigen from a Toxin-Producing Strain of Hemophilus Pertussis

[摘要] The serological characteristics of a toxin producing strain of H. pertussis , # 29, are compared with a typical phase I strain, # 18-323. A water extract of strain # 29 organisms, heated at 56 C for 45 minutes, contains a protective antigen against a typical phase I strain, # 18-323, the challenge organism in the mouse protection test. The protective antigen can be partially separated from the heated, non-toxic, water extract by fractionation with methanol-water mixtures under controlled conditions of pH, ionic strength and temperature.

[学科分类] 生物科学（综合）