The in Vivo Activity of in Vitro Immunized Mouse Thymocytes

[摘要] “Cortisone resistant” CBA mouse thymocytes activated in vitro against allogeneic spleen cells and capable of lysing allogeneic target cells in vitro were assayed for their capacity to mount cell-mediated immune responses in vivo. These in vitro activated thymocytes (against H-2d alloantigens) induced the rejection of BALB/c (H-2d) skin grafts from T cell-depleted CBA mice. 107 or more cytotoxic lymphocytes (CL) caused rejection within 12 days from T cell-depleted mice but failed to reject grafts on lethally irradiated mice.As compared to normal “cortisone resistant” thymocytes, in vitro activated CL were inefficient in causing splenomegaly on injection into neonatal (CBA × BALB/c)F1 mice. However, CL mounted twice the mitotic response of “cortisone resistant” thymocytes when injected into adult lethally irradiated (CBA × BALB/c)F1 mice. The results indicate that there exists no direct relationship between the capacity of thymocytes to act as cytotoxic effector cells and to induce splenomegaly in neonatal F1 hybrid mice.

[学科分类] 生物科学（综合）