Differentiation of Type 1 and Type 2 Herpes Simplex Virus by in Vitro Stimulation of Immune Lymphocytes

[摘要] In a previous report we demonstrated that lymphocytes from rabbits immunized with herpes simplex virus (HSV) or vaccinia virus could be stimulated in vitro by the homologous but not heterologous virus (1). These observations indicated that the lymphocyte stimulation technique, believed to be an in vitro correlate of cell-mediated immunity (2), could distinguish between antigenically unrelated viruses. Recently it has been shown (3, 4) that HSV could be differentiated by serologic techniques into two closely related antigenic types (HSV-1 and HSV-2). The present study was initiated to determine whether the two types of HSV also could be distinguished by the in vitro lymphocyte stimulation technique.Materials and Methods. The methods employed in the present study have been described in detail elsewhere (1). HSV-1 (strain VR3) and HSV-2 (strain MS) were prepared and assayed in primary rabbit kidney cells grown in the presence of 5% rabbit serum (3, 5).

[学科分类] 生物科学（综合）