Serologic Cross-Reactivity Between H-2 and HL-A Antigens

[摘要] Soluble HL-A antigen extracted from cultured human lymphoid cells (RPMI 1788, HL-A 2, 10, 7, W14) by the 3M KCl method was injected into rabbits. The rabbit anti-HL-A sera contained antibodies against all the HL-A specificities present on the immunogen. Moreover, these same xenoantibodies reacted also with certain murine lymphocytes bearing distinct H-2 specificities in the direct complement dependent lymphocytotoxic test. Absorption studies confirmed this selected reactivity and led to the conclusion that mainly antigenic determinants of the D region of H-2d and the K region of H-2b are involved in this cross-reactivity. Rabbit anti-HL-A sera absorbed with cells of different mouse strains were tested against a large panel of human lymphocytes from unrelated donors for remaining cytotoxic activity. The results indicate that absorption with cells of the genotypes H-2b (KbDb) and H-2a (KkDd) remove antibodies against determinants of the first segregant series (cross-reacting with HL-A 2 and 10).These data suggest a specific cross-reactivity between human and murine histocompatibility antigens and suggest a certain structural homology between these cell surface antigens and perhaps even a similar organization of the genetic fine structure of the HL-A and H-2 systems.

[学科分类] 生物科学（综合）