Immune Hemolysis

[摘要] Erythrocytes of 19 sheep comprising 3 genetic types were studied for susceptibility to lysis by constant amounts of rabbit hemolysin and human complement. The sheep were classified as low K+ (LK) or high K+ (HK) by red cell K+ concentrations and then as antigenic types LL, LM or MM by detection of L and M erythrocyte antigens with specific antisera. The three genetic types of red cells consistently yielded differing levels of hemolysis in the following order of sensitivity: LK (LL) τ; LK (LM) τ; HK (MM). These differences could not be overcome by higher doses of antibody, were observed with both IgM and IgG hemolysin, and were not attributable to differing rates of hemolysis. A high K+ medium enhanced immune lysis of HK (MM) and LK (LM) cells but not LK (LL) cells. When the relationship of immune lytic potential of red cells to their sphericity and rigidity was examined by microscopic morphology, osmotic fragility and cellular deformability, heterogeneity was encountered within genetic types. The practical importance of these genetic differences in sheep erythrocytes for laboratories performing complement assays as a clinical service is demonstrated by comparative CH50 titers on representative human sera.

[学科分类] 生物科学（综合）