Precipitin Production in Chickens

[摘要] The effects on the chicken precipitin system of a Hofmeister series of sodium salts were investigated. With any particular ionic species there were differences in the amounts of specific precipitation on varying the ionic strength, and at any one ionic strength there were differences in varying the ionic species. Certain characteristic patterns emerged. At low ionic strength (up to 0.26) precipitation was larger in the iodide medium than in any of the other ionic media. On increasing the ionic strength above 0.26 precipitation in the iodide medium decreased. This inhibition of the reaction was even more striking in the thiocyanate medium. These results suggested that a certain amount of anion binding by the antibody-antigen reactants increased precipitation but that greater amounts of binding decreased precipitation.With anions such as chloride and sulfate precipitation increased with each increase of ionic strength. In fact the maximal precipitation for the chicken system in the various sodium salts occurred at high ionic strengths (about 1.5) of chloride and sulfate. These results in turn suggested that a salting out of soluble antibody-antigen aggregates is an important condition for obtaining complete precipitation with the chicken system. Additional data which also demonstrated the operation of a salting out effect was obtained by reacting the chicken precipitin system in potassium phosphate buffers of varied concentration.

[学科分类] 生物科学（综合）