Microdetermination of Shigella Hemagglutinins in Human and Rabbit Sera with Monovalent and Polyvalent Antigens

[摘要] S. flexneri antibodies in rabbit and human serum specimens were demonstrated using a microadaptation of the indirect bacterial hemagglutination test. Although three types of antigen preparations are suitable for detecting hemagglutinins, a polyvalent preparation is of most interest since it has the greatest potential practical application. Comparisons with monovalent antigens, prepared from stock cultures and organisms from patients, showed the polyvalent preparation to be sensitive and specific.Hemagglutinins reached peak titers on the 5th to 14th day of illness in infants and children with shigellosis. Evidence is presented indicating that effective antimicrobial therapy of shigellosis depresses the specific hemagglutinin response, making it necessary to interpret hemagglutination results in the light of the therapy patients have received.

[学科分类] 生物科学（综合）