The Use of a Water-Soluble Carbodiimide as a Coupling Reagent in the Passive Hemagglutination Test

[摘要] A simplified method is described for coupling proteins to red cells using the water-soluble carbodiimide, 1 - ethyl - 3 - (3 - dimethylaminopropyl) carbodiimide hydrochloride. The procedure is simpler than BDB coupling and the sensitized cells are more stable than those in the BDB system. The data indicated a marked similarity in the specificity and sensitivity of ECDI-and BDB-hemagglutination.

[学科分类] 生物科学（综合）