Purification of Rabies Antibodies in Horse Serum and Diagnostic Importance of the Fluorescent Antibody Technique

[摘要] 1. 1. Purified horse rabies antibody has been prepared by the isoelectric fractional alcohol precipitation method of Nichol and Deutsch, with a yield of 54%. Neutralizing antibody in the final preparation is found mainly in the γ-2-G fraction.2. 2. Modifications of the method of Coons were used for conjugating the purified antirabies horse antibody with fluorescein isocyanate. Staining of brain smears from rabies-infected dogs and mice with fluorescent antibody showed the diagnostic usefulness of this method in a type of communicable disease in which prompt information is most urgent.

[学科分类] 生物科学（综合）