Studies of Polyoma Virus

[摘要] Titration of partially purified cell-free virus in newborn hamsters revealed that the dose response curves for tumor or antibody formation is shallower than would be expected if one particle were sufficient to induce a tumor or trigger the events that lead to antibody response. Hemagglutination-inhibiting or complement-fixing antibody reached peak levels between 30 and 70 days and was a more sensitive indicator of virus than tumor response.Hamsters with tumors at multiple sites developed high antibody titers. Hamsters with tumors at one site, particularly the site of injection, occasionally lacked demonstrable antibody. Such a condition could also be produced following simultaneous injection of newborn hamsters with virus and serum at different sites.Virus was demonstrated in 2 of 14 tumor homogenates by injection into mice, and in 7 of 22 tumors by injection of fluids or cells from tumor cell cultures into newborn hamsters. Most isolations were from homogenates or cultures of tumors from animals with moderate and high antibody titers. Hamsters injected with live cells from tumor cell cultures frequently developed invasive tumors at the site of injection but no antibody. These experiments lead us to believe that (1) lack of demonstrable antibody in some hamsters with tumors is due to virus multiplication that is inadequate to stimulate a good antibody response, (2) virus isolation is more probable from animals in which virus multiplied extensively and which in turn developed high antibody levels, and (3) tumor formation may not be related to the extent of virus multiplication in the hamster.

[学科分类] 生物科学（综合）