Purification of Q Fever Rickettsiae by Density-Gradient Sedimentation

[摘要] A rapid method for isolation and purification of Q fever rickettsiae is described. It consists of differential centrifugation of homogenates of infected yolk sacs or chick embryo tissue in M/1 KCl followed by sedimentation in continuous linear sucrose or glycerin gradients. Adequate yields of electron microscopically pure rickettsiae were obtained.

[学科分类] 生物科学（综合）