Immunochemical Analysis of Human Serum and Its Fractions

[摘要] The fraction of human serum precipitated by 45% saturated ammonium sulphate has been submitted to immunochemical analysis. “Fraction 45” was separated by ammonium sulfate in the presence of phosphate buffer into seven main subfractions. A number of immune serums were prepared against a part of these subfractions and against other fractions of human serum. The reactions in gelled media (simple diffusion in tubes) of these immune serums with Fraction 45, its subfractions (brought to the same nitrogen concentration), and other fractions, were carried out and analyzed. The identification of the precipitation zones of sufficient density and penetration given by the same antigen in these multiple reactions was mainly carried out by a procedure previously described which involves only the comparison of the tubes. Fifteen distinct antigens and a family of at least three antigens with similar properties of solubility and immunogenic power, making a total of 18 antigens could be distinguished. It was demonstrated that the 18 antigens found in Fraction 45 were different from the seven antigens already described in the fraction soluble in 65% saturated ammonium sulfate. Consequently, the number of antigenic components of human serum that we have described to date is not less than 25—the components of certain fractions remaining to be described. Unispecific immune serums against six major components of Fraction 45 and two major components of other fractions were prepared.

[学科分类] 生物科学（综合）