Serologic Studies of Purified Staphylococcal Coagulase with Special Reference to Fluorescence Microscopy

[摘要] Rhodamine isothiocyanate-labeled antibody prepared against electrophoretically purified coagulase was employed after absorption with a coagulase-negative strain of Staphylococcus, to differentiate between coagulase-positive and coagulase-negative staphylococci. By application of this reagent, a close association between coagulase production and the staining reaction was observed, but absolute specificity could not be established. Some relationship between the bacteriophage group of staphylococcal strains and the antigenic specificity of free coagulase produced by the corresponding strains was observed in cross-inhibition experiments. Double diffusion precipitin analysis revealed heterogeneity of the antiserum prepared against highly purified coagulase.

[学科分类] 生物科学（综合）