The Influence of Removing Lymphoid Tissue on the Persistence of Tolerance of Skin Allografts in Mice

[摘要] To determine what influence removing lymphoid tissue had on the survival of foreign grafts on tolerant animals, we lymphnodectomized and/or splenectomized tolerant A-strain mice, bearing CBA skin grafts of long-standing. The results indicated that these procedures had no demonstrable effect on the survival of the tolerated graft unless the hosts were rechallenged with a second CBA graft—a procedure which was routinely carried out 60 days after the ablative operation. After regrafting, 24 of 42 animals (57%) devoid of their axillary and brachial nodes rejected their original grafts and 16 (38%) rejected both grafts as compared to only two of 25 (8%) which rejected their original graft, and none both, in the corresponding sham-operated groups. Unilateral lymphnodectomy had significantly less influence on graft survival than bilateral lymphnodectomy. Moreover, the detrimental influence of bilateral lymphnodectomy on graft survival could be partially reversed by inoculating a coarse cell suspension of some or all of the removed nodes back into the animals from which they were derived. Finally, splenectomy, even when combined with lymphnodectomy, had little influence on graft survival.

[学科分类] 生物科学（综合）